Testing the Toxicity of Stachybotrys chartarum in Indoor Environments—A Case Study

Infestation of interior walls buildings with fungal mould is a reason for health concern which exacerbated in energy-efficient that limit air circulation. Both mycological and mycotoxicological studies are needed to determine the potential hazards residents. In this paper, rare case occurrence Stachybotrys chartarum an apartment building Lubuskie Province Poland has been described. Isolated as major constituent mixed mycobiota, its specific relevance still needs be carefully analyzed biochemical aptitude synthesis mycotoxins may expressed at different levels. Therefore, ecotoxicological tests were performed using two bioindicators: Dugesia tigrina Girard Daphnia magna Straus. D. was used first time examine toxicity S. chartarum. The showed strain belonged third fourth classes according Liebmann’s classification. moderately toxic on Potato Dextrose Agar (PDA) culture medium (toxicity class III), slightly Malt Extract (MEA) IV). Toxicity additionally tested by instrumental analytical methods (LC-MS/MS). This method allowed identification 13 metabolites (five reported eight unspecific metabolites). Spirocyclic drimanes detected considerable quantities (ng/g); higher concentration observed stachybotryamide (109,000 PDA 62,500 MEA) lower stachybotrylactam (27,100 46,300 MEA). explain result through bioindicators. Highly compounds such satratoxins not found sample. confirms applicability bioindicators, also show mutual compatibility, suitable tools assess moulds.